Interaction Details


Details of experiments between P0DTC9 and Q8IUC4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA

Protein B

Protein: RHPN2
Fragment: 506-606
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.75
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.24
PUBMED ID: 36115835