Interaction Details


Details of experiments between P0DTC4 and Q8N3R9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV

Protein B

Protein: MPP5
Fragment: 238-336
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.01
PUBMED ID: 36115835