Interaction Details


Details of experiments between P0DTC4 and P55196
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV

Protein B

Protein: MLLT4
Fragment: 1002-1097
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.1
PUBMED ID: 36115835