Interaction Details


Details of experiments between P0DTC4 and O14745
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV

Protein B

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.76
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV

Protein B

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835