Interaction Details


Details of experiments between P0DTC3 and Q7Z6J2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Protein B

Protein: GRASP
Fragment: 96-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.83
PUBMED ID: 36115835