Interaction Details


Details of experiments between P0DTC3 and Q5T5U3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Protein B

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.75
PUBMED ID: 36115835