Interaction Details


Details of experiments between P0DTC3 and Q07157
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Protein B

Protein: TJP1
Fragment: 184-264
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.56
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Protein B

Protein: TJP1
Fragment: 14-113
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Protein B

Protein: TJP1
Fragment: 415-516
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835