Interaction Details


Details of experiments between P08670 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: VIM
Fragment: 1-466
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-MBP-SNX27-his6
Experiment method: nHU-WB_Jurkat
Details of affinity fitting: Hill equation (8 point titration) h=1.9
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 7.1
PUBMED ID: 36542723

Experiment series 2

Protein A

Protein: VIM
Fragment: 1-466
Construct: endogenous protein (SH-SY5Y)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.96
Immobilized partner concentration (10-6M): 10
P value: 12.55
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 6.38
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 3

Protein A

Protein: VIM
Fragment: 1-466
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27
Average holdup BI: 0.95
Immobilized partner concentration (10-6M): 10
P value: 12.82
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 6.32
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 4

Protein A

Protein: VIM
Fragment: 1-466
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.95
Immobilized partner concentration (10-6M): 10
P value: 14.53
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
Measured pKd: 6.24
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 5

Protein A

Protein: VIM
Fragment: 1-466
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.91
Immobilized partner concentration (10-6M): 10
P value: 11.02
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 6
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))