Interaction Details


Details of experiments between P07550 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ADRB2
Fragment: 404-413
Site: PBM
Sequence: RNCSTNDSLL
Construct: Biotin-Ahx-RNCSTNDSLL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.6
PUBMED ID: 36115835