Interaction Details


Details of experiments between P06463 and P29074
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: PTPN4
Fragment: 513-603
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 13.3
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.62
PUBMED ID: 36115835