Interaction Details


Details of experiments between P06463 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 13.3
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.33
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.01
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 121.2
Standard deviation of dissociation constant (10-6M): 6.5
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.92
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.25
Immobilized partner concentration (10-6M): 43
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Sequence: RLQRRRETQV
Construct: biotin-ttds-RLQRRRETQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 43
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: HPV18-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: RLQRRRETQV
Construct: Biotin-ttds-RLQRRREpTQV

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835