Interaction Details


Details of experiments between P06427 and Q00013
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL

Protein B

Protein: MPP1
Fragment: 69-153
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.79
PUBMED ID: 36115835