Interaction Details


Details of experiments between P03409 and Q9UPQ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.57
Holdup BI standard deviation: 0.11
Normalized holdup BI: 0.61
Normalized holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 15
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.1
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 8.02
Standard deviation of dissociation constant (10-6M): 2.95
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.1
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.65
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 5.06
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: Biotin-ado-ado-SEKHFRETEV

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Normalized holdup BI: 0.6
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.98
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: HTLV1-TAX1
Fragment: 344-353
Site: PBM
Sequence: SEKHFRETEV
Construct: biotin-ttds-SEKHFRETEV

Protein B

Protein: PDZRN3
Fragment: 405-515
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 19
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835