Interaction Details


Details of experiments between P03146 and Q13425
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC

Protein B

Protein: SNTB2
Fragment: 112-199
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.58
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.