Interaction Details


Details of experiments between P03126 and Q5TCQ9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.9
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 7
Measured pKd: 5.91
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Normalized holdup BI: 0.84
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.56
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.68
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.75
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.31
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Normalized holdup BI: 0.73
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.25
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 847-941
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 7
Measured pKd: 3.96
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 1-114
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 1014-1103
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 7
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 572-655
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: MAGI3
Fragment: 716-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 7
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 1014-1103
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 572-655
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 716-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: MAGI3
Fragment: 847-941
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835