Interaction Details


Details of experiments between P03126 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Normalized holdup BI: 0.6
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.98
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.54
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.87
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 7
Measured pKd: 4.85
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Normalized holdup BI: 0.48
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.75
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835