Interaction Details


Details of experiments between P03126 and Q9NUP9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 6
Measured pKd: 4.29
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Holdup BI standard deviation: 0
Normalized holdup BI: 0.16
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.04
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.94
PUBMED ID: 36115835