Interaction Details

Details of experiments between P03126 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: HPV16E6
Fragment: 139-149
Site: 14-3-3_pThr156
Modification: (TPO)156
Sequence: RTRRETQL--
Construct: SSRTRRE(pThr)QL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: -0.26

Holdup BI standard deviation: 1.41

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 134

P value: 0.16

Experiment method: HU Multiplex

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-02-17

Number of measurements: 4

The affinity was below the detection threshold of the assay.

DOI: 10.1101/2022.12.08.519103

Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))