Interaction Details


Details of experiments between P00519 and Q9Y2K7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: KDM2A
Fragment: 1-1162
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.03
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: KDM2A
Fragment: 545-559
Site: PxxP_1
Sequence: PAPRLTPVRPAAASP
Construct: Bio Ado Ado-PAPRLTPVRPAAASP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680