Interaction Details


Details of experiments between P00519 and Q8WUM4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: PDCD6IP
Fragment: 1-868
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.27
Immobilized partner concentration (10-6M): 10
P value: 8.57
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.57
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: PDCD6IP
Fragment: 746-760
Site: PxxP_1
Sequence: TMPPTKPQPPARPPP
Construct: Bio Ado Ado-TMPPTKPQPPARPPP-CONH2
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.93
PUBMED ID: 38995680