Interaction Details


Details of experiments between P00519 and O95785
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WIZ
Fragment: 157-171
Site: PxxP_3
Sequence: LPAQPPPLAPFRRVF
Construct: Bio Ado Ado-LPAQPPPLAPFRRVF-CONH2
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Protein B

Protein: WIZ
Fragment: 1-1651
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.39
Immobilized partner concentration (10-6M): 10
P value: 3.07
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WIZ
Fragment: 1135-1149
Site: PxxP_1
Sequence: PLAKKLPPPPGSPLG
Construct: Bio Ado Ado-PLAKKLPPPPGSPLG-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WIZ
Fragment: 1164-1178
Site: PxxP_2
Sequence: FPGLAAPSLPKKLKP
Construct: Bio Ado Ado-FPGLAAPSLPKKLKP-CONH2
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3

Protein B

Protein: WIZ
Fragment: 9-23
Site: PxxP_4
Sequence: LAAPDRPQGPERLPG
Construct: Bio Ado Ado-LAAPDRPQGPERLPG-CONH2
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680