Interaction Details


Details of experiments between O96018 and Q14517
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: APBA3
Fragment: 390-479
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT1
Fragment: 4579-4588
Site: PBM
Sequence: PLDSQQHTEV
Construct: biotin-ado-ado-PLDSQQHTEV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.