Interaction Details


Details of experiments between O95544 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NADK
Fragment: 43-52
Site: 14-3-3_pSer48
Modification: (SEP)48
Sequence: KSRSLSASPA
Construct: KSRSL(pSer)ASPA (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.86
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 129
P value: 1.81
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.69
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: NADK
Fragment: 43-52
Site: 14-3-3_pSer48
Modification: (SEP)48
Sequence: KSRSLSASPA
Construct: KSRSL(pSer)ASPA (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.8
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 3.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.48
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))