Interaction Details


Details of experiments between O95049 and Q9UPQ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TJP3
Fragment: 910-919
Site: PBM
Sequence: GYDWGPATDL
Construct: Biotin-Ahx-GYDWGPATDL

Protein B

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.69
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDZRN3
Fragment: 1057-1066
Site: PBM
Sequence: YNSFLSVTTV
Construct: Biotin-Ahx-YNSFLSVTTV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.