Interaction Details

Details of experiments between O95049 and Q9UBN1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CACNG4
Fragment: 318-327
Site: PBM
Sequence: SMLNRRTTPV
Construct: biotin-ado-ado-SMLNRRTTPV

Average holdup BI: -0

Holdup BI standard deviation: 0.01

Normalized holdup BI: -0

Normalized holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.