Interaction Details

Details of experiments between O95049 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: TJP3
Fragment: 910-919
Site: PBM
Sequence: GYDWGPATDL
Construct: Biotin-Ahx-GYDWGPATDL

Protein B

Protein: MAGI1
Fragment: 1-106
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: 0.02

Normalized holdup BI: 0.02

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

P value: 0

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 2