Interaction Details


Details of experiments between O95049 and Q6UXZ0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 36115835