Interaction Details


Details of experiments between O95049 and P0DTC9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TJP3
Fragment: 1-109
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TJP3
Fragment: 189-274
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.14
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.12
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835