Interaction Details


Details of experiments between O95049 and P03146
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TJP3
Fragment: 1-109
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.06
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: TJP3
Fragment: 189-274
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.16
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: TJP3
Fragment: 373-482
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.06
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.