Interaction Details

Details of experiments between O75970 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MPDZ
Fragment: 998-1105
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.13

Holdup BI standard deviation: 0.32

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

Measured pK_d: 3.94

PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MPDZ
Fragment: 1141-1246
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.04

Holdup BI standard deviation: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MPDZ
Fragment: 120-230
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.01

Holdup BI standard deviation: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MPDZ
Fragment: 1325-1441
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.01

Holdup BI standard deviation: 0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MPDZ
Fragment: 1464-1572
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.01

Holdup BI standard deviation: 0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0

Normalized holdup BI: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0.01

Holdup BI standard deviation: 0.09

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MPDZ
Fragment: 1722-1810
Site: PDZ_11
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0.05

Holdup BI standard deviation: 0.07

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.04

Holdup BI standard deviation: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: MPDZ
Fragment: 1982-2070
Site: PDZ_13
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0.06

Holdup BI standard deviation: 0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: MPDZ
Fragment: 246-342
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0

Holdup BI standard deviation: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: MPDZ
Fragment: 371-467
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.08

Holdup BI standard deviation: 0.15

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: MPDZ
Fragment: 548-634
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0.03

Holdup BI standard deviation: 0.08

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 5

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: -0

Normalized holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Average holdup BI: 0.06

Holdup BI standard deviation: 0.09

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835