Interaction Details


Details of experiments between O75970 and Q9NR12
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDLIM7
Fragment: 448-457
Site: PBM
Sequence: LCKSHAFSHV
Construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.07
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDLIM7
Fragment: 448-457
Site: PBM
Sequence: LCKSHAFSHV
Construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDLIM7
Fragment: 448-457
Site: PBM
Sequence: LCKSHAFSHV
Construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835