Interaction Details


Details of experiments between O75970 and Q12951
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FOXI1
Fragment: 369-378
Site: PBM
Sequence: VLYPREGTEV
Construct: biotin-ado-ado-VLYPREGTEV
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FOXI1
Fragment: 369-378
Site: PBM
Sequence: VLYPREGTEV
Construct: biotin-ado-ado-VLYPREGTEV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.49
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FOXI1
Fragment: 369-378
Site: PBM
Sequence: VLYPREGTEV
Construct: biotin-ado-ado-VLYPREGTEV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.