Interaction Details


Details of experiments between O75717 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: WDHD1
Fragment: 1-1129
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 1-541
Construct: avi(biotin)-his6-MBP-TEVsite-SNX27
Average holdup BI: -0.17
Immobilized partner concentration (10-6M): 10
P value: 1.29
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 2

Protein A

Protein: WDHD1
Fragment: 1-1129
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
P value: 0.58
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: WDHD1
Fragment: 1-1129
Construct: endogenous protein (SH-SY5Y)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: -0.17
Immobilized partner concentration (10-6M): 10
P value: 2.1
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 4

Protein A

Protein: WDHD1
Fragment: 1-1129
Construct: endogenous protein (Jurkat)

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 10
P value: 2.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-27
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 6))

Experiment series 5

Protein A

Protein: WDHD1
Fragment: 906-924
Construct: Biotin-Ahx-SSQGRVNPFKVSASSKEPA-CONH2

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous proteins (Jurkat)
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.25
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 1.78

Experiment series 6

Protein A

Protein: WDHD1
Fragment: 906-924
Construct: Biotin-Ahx-SSQGRVNPFKVSASSKEPA-CONH2

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
P value: 1.17
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-09-01
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch4; Ncontrols = 18; Nsamples = 6; Npreys = 5752; threshold curvature: 1.66