Interaction Details


Details of experiments between O75096 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LRP4
Fragment: 1896-1905
Site: PBM
Sequence: ERKLSSESQV
Construct: biotin-ttds-ERKLSSESQV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.48
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 4.75
PUBMED ID: 36115835