Interaction Details


Details of experiments between O75044 and P27348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SRGAP2
Fragment: 422-431
Site: 14-3-3_pSer427
Modification: (SEP)427
Sequence: VKSTVSETFM
Construct: VKSTV(pSer)ETFM (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.23
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 4.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.37
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: SRGAP2
Fragment: 849-858
Site: 14-3-3_pSer854
Modification: (SEP)854
Sequence: TFRSDSHGLS
Construct: TFRSD(pSer)HGLS (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 0.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))