Interaction Details

Details of experiments between O60880 and Q9BRQ0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: SH2D1A
Fragment: 1-128
Construct: endogenous proteins (Jurkat)

Protein B

Protein: PYGO2
Fragment: 57-86
Construct: biotin-ahx-HHSSNWLRNPFKVLDKNQEPALWKQLIK-CONH2

Average holdup BI: 0.04

Immobilized partner concentration (10^-6M): 10

P value: 0.82

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic equation

Date of measurement: 2024-09-20

Number of measurements: 6

The affinity was below the detection threshold of the assay.

Experimental Details

Batch = Helsinki_batch8; Ncontrols = 24; Nsamples = 6; Npreys = 6577; threshold curvature: 1.87