Interaction Details


Details of experiments between O60880 and O75717
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SH2D1A
Fragment: 1-128
Construct: endogenous proteins (Jurkat)

Protein B

Protein: WDHD1
Fragment: 906-924
Construct: Biotin-Ahx-SSQGRVNPFKVSASSKEPA-CONH2
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
P value: 0.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 1.78

Experiment series 2

Protein A

Protein: SH2D1A
Fragment: 1-128
Construct: endogenous proteins (Jurkat)

Protein B

Protein: WDHD1
Fragment: 906-924
Construct: Biotin-Ahx-SSQGRVNPFKVSASSKEPA-CONH2
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 10
P value: 1.16
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-09-01
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch4; Ncontrols = 18; Nsamples = 6; Npreys = 5752; threshold curvature: 1.66