Interaction Details


Details of experiments between O60825 and P31947
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0
Holdup BI standard deviation: 0.32
Immobilized partner concentration (10-6M): 113
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.02
Holdup BI standard deviation: 0.35
Immobilized partner concentration (10-6M): 142
P value: 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.2
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 113
P value: 1.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.2
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 142
P value: 1.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.54
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 113
P value: 4.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.51
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 6.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.86
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.21
Holdup BI standard deviation: 0.5
Immobilized partner concentration (10-6M): 111
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.27
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 111
P value: 2.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.52
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.55
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 4.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.05
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))