Interaction Details


Details of experiments between O60825 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.56
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 129
P value: 4.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.45
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 134
P value: 1.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.78
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.65
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 129
P value: 4.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.16
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.45
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 134
P value: 3.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.78
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.74
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 5.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.33
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.66
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 7.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.18
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.58
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 115
P value: 1.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.1
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.58
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 115
P value: 3.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.09
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.68
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 115
P value: 4.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.29
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))