Interaction Details


Details of experiments between O60825 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PFKFB2
Fragment: 461-470
Site: 14-3-3_pSer466
Modification: (SEP)466
Sequence: RMRRNSFTPL
Construct: RMRRN(pSer)FTPL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.33
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 102
P value: 2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.43
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 102
P value: 3.47
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.86
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: PFKFB2
Fragment: 478-487
Site: 14-3-3_pSer483
Modification: (SEP)483
Sequence: RPRNYSVGSR
Construct: RPRNY(pSer)VGSR (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.39
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 134
P value: 2.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.69
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.68
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 5.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.2
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: PFKFB2
Fragment: 470-479
Site: 14-3-3_pThr475
Modification: (TPO)475
Sequence: LSSSNTIRRP
Construct: LSSSN(pThr)IRRP (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.59
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 102
P value: 7.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.14
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))