Interaction Details


Details of experiments between O60711 and Q5T5U3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LPXN
Fragment: 377-386
Site: PBM
Sequence: QPCFNKLFPL
Construct: biotin-ado-ado-QPCFNKLFPL

Protein B

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.