Interaction Details

Details of experiments between O60307 and P03146
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MAST3
Fragment: 948-1039
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HBV-Hbc
Fragment: 174-183
Site: PBM
Sequence: RRSQSRESQC
Construct: biotin-(PEG)3-RRRRSQSRESQC

Average holdup BI: -0.05

Holdup BI standard deviation: 0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.