Interaction Details

Details of experiments between O43516 and P30291
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: WIPF1
Fragment: 1-503
Construct: endogenous proteins (Jurkat)

Protein B

Protein: WEE1
Fragment: 228-247
Construct: Biotin-Ahx-RQTPQVNINPFTPDSLLLHS-CONH2

Average holdup BI: -0.15

Immobilized partner concentration (10^-6M): 10

P value: 0.5

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic equation

Date of measurement: 2024-04-11

Number of measurements: 6

The affinity was below the detection threshold of the assay.

Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.12