Interaction Details


Details of experiments between O43464 and Q05586
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTRA2
Fragment: 355-458
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN1
Fragment: 929-938
Site: PBM
Sequence: LSDPSVSTVV
Construct: Biotin-Ahx-LSDPSVSTVV
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835