Interaction Details


Details of experiments between O43464 and O15169
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTRA2
Fragment: 355-458
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AXIN1
Fragment: 333-347
Site: Internal_PBM
Construct: Biotin-GSGSGS-TLSLTDSSVDGIPPYR-CONH2
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835