Interaction Details


Details of experiments between O43426 and Q9UBC2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: EPS15L1
Fragment: 1-864
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.78
Immobilized partner concentration (10-6M): 10
P value: 11.15
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
Measured pKd: 5.54
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: EPS15L1
Fragment: 1-864
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.61
Immobilized partner concentration (10-6M): 10
P value: 21.24
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
Measured pKd: 5.2
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72