Interaction Details


Details of experiments between O43426 and Q9NZM3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: ITSN2
Fragment: 1-1697
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 10
P value: 5.78
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
Measured pKd: 5.12
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: ITSN2
Fragment: 1-1697
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 10
P value: 15.66
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
Measured pKd: 4.98
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72