Interaction Details


Details of experiments between O43426 and Q86XP1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous protein (Jurkat)

Protein B

Protein: DGKH
Fragment: 1211-1220
Site: PBM
Sequence: LGRSTPQSEV
Construct: biotin-ttds-LGRSTPQSEV
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 10
P value: 1.08
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: DGKH
Fragment: 1-1220
Construct: endogenous proteins (Jurkat)
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72

Experiment series 3

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: DGKH
Fragment: 1-1220
Construct: endogenous proteins (Jurkat)
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 10
P value: 1.36
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54