Interaction Details


Details of experiments between O43426 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous protein (Jurkat)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.55
Immobilized partner concentration (10-6M): 10
P value: 5.31
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.09
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.55
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.89
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.28
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.22
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.15
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.59
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous proteins (Jurkat)
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 10
P value: 0.35
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 2.72

Experiment series 12

Protein A

Protein: SYNJ1
Fragment: 1390-1421
Construct: Biotin-Ahx-RSSPNPFITGLTRTNPFSDRTAAPGNPFRAKS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 1-803
Construct: endogenous proteins (Jurkat)
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.12
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2023-12-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch5; Ncontrols = 12; Nsamples = 6; Npreys = 6006; threshold curvature: 1.54

Experiment series 13

Protein A

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680