Interaction Details


Details of experiments between O43426 and P55345
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SYNJ1
Fragment: 1533-1547
Site: PxxP_10
Sequence: LPSRRPPPPPVPLLP
Construct: Bio Ado Ado-LPSRRPPPPPVPLLP-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: SYNJ1
Fragment: 1056-1070
Site: PxxP_1
Sequence: ISEGPVPSLPIRPSR
Construct: Bio Ado Ado-ISEGPVPSLPIRPSR-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.69
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: SYNJ1
Fragment: 1-1573
Construct: endogenous protein (Jurkat)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Immobilized partner concentration (10-6M): 10
P value: 2.87
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: SYNJ1
Fragment: 1059-1073
Site: PxxP_2
Sequence: GPVPSLPIRPSRAPS
Construct: Bio Ado Ado-GPVPSLPIRPSRAPS-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: SYNJ1
Fragment: 1096-1110
Site: PxxP_3
Sequence: QKDPAQPLEPKRPPP
Construct: Bio Ado Ado-QKDPAQPLEPKRPPP-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: SYNJ1
Fragment: 1104-1118
Site: PxxP_4
Sequence: EPKRPPPPRPVAPPT
Construct: Bio Ado Ado-EPKRPPPPRPVAPPT-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: SYNJ1
Fragment: 1114-1128
Site: PxxP_5
Sequence: VAPPTRPAPPQRPPP
Construct: Bio Ado Ado-VAPPTRPAPPQRPPP-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: SYNJ1
Fragment: 1190-1204
Site: PxxP_6
Sequence: GYSTARPTIPPRAGV
Construct: Bio Ado Ado-GYSTARPTIPPRAGV-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: SYNJ1
Fragment: 1278-1292
Site: PxxP_7
Sequence: PNLETPPQPPPRSRS
Construct: Bio Ado Ado-PNLETPPQPPPRSRS-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: SYNJ1
Fragment: 1371-1385
Site: PxxP_8
Sequence: SCMPTMPPIPARSQS
Construct: Bio Ado Ado-SCMPTMPPIPARSQS-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: SYNJ1
Fragment: 1407-1421
Site: PxxP_9
Sequence: SDRTAAPGNPFRAKS
Construct: Bio Ado Ado-SDRTAAPGNPFRAKS-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680